C1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Mouse Hybridoma
• packaging: tube of 5 μg 99061825-DNA-5UG; pkg of vial of cells 99061825-1VL
• ImmunoDon: (C57BL/6xBALB/c)F1
• Myeloma: P3x63Ag8.653
• Reactivity: Anti-HLA DQ2
• technique(s): cell culture | mammalian: suitable
• isotype: IgM
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse x Mouse hybridoma

Cell Line Description

The hybridoma C1 was produced from a (C57Bl/6xBALB/c)F1 mouse immunised with a single i.p. injection of the lymphoblastoid cell line Co and Bordetella pertussis organisms. The B cell line Co was expressing HLA A1, B8, C7, DR3 and DQ2. The mouse spleen was removed 3 days after immunisation and fused with the myeloma P3x63Ag8.653. C1 monoclonal antibodies were shown to react with cells expressing the class II antigen DQw2. The hybridoma is also known as COX IA2. C1 has been analysed in the International Histocompatibility Workshop 10 (IOW3105) and 12 (12W593).

Immunogen

B cell line Co, HLA A1,B8 C7, DR3,DQ2

Application

Immunofluorescence, cytotoxicity

Culture Medium

RPMI 1640 + 2mM glutamine + 1mM NaP + 10% FBS

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C

Other Notes

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