Lu6

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Mouse Hybridoma
• packaging: tube of 5 μg 99043004-DNA-5UG; pkg of vial of cells 99043004-1VL
• ImmunoDon: (C57BL/6 x BALB/c)F1
• Myeloma: P3 x 63 Ag8.653
• Reactivity: Anti-HLA DRII
• technique(s): cell culture | mammalian: suitable
• isotype: IgG3
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse x Mouse hybridoma

Cell Line Description

Lu6 was established from a (C57BL/6 X BALB/c) F1 mouse immunised with a i.p. injection of the lymphoblastoid cell line Lutz Cor and B-pertussis adjuvant. The B cell line was expressing HLA A3, 2; B18, 5; C5; DR3,II. Mice spleen was removed 3 days after immunisation and fused with the myeloma P3x63Ag8.653. Lu6 monoclonal antibodies were shown to react with HLA DRII. Lu6 is also known as LC4A6. It has been described in the International Histocompatibility Workshop II (IIW0912) and 12 (12W539). The cells have been eradicated from mycoplasma using Cibrobay before being deposited at ECACC.

Immunogen

B cell line Lutz Cor HLA A3, 2; B18,5; C5; DR3,II

Application

Tumour studies

Culture Medium

RPMI 1640 + 2 mM glutamine + 1mM sodium pyruvate + 10% FBS

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.