S2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Mouse Hybridoma
• packaging: tube of 5 μg 99043002-DNA-5UG; pkg of vial of cells 99043002-1VL
• ImmunoDon: BALB/c X C57bL/6)F1,spleen
• Myeloma: P3x63 Ag6.653
• Reactivity: Anti-HLA DR13
• technique(s): cell culture | mammalian: suitable
• isotype: IgM
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

P3 x 63 Ag8.653

Cell Line Description

The hybridoma line S2 has been obtained by fusion of the myeloma P3x63 Ag6.653 and spleen cells from (C57 BL/6 x BALB/c) F1 female mice after a single i.p. injection of lymphoblastoid cells. These lymphoblastoid cells were DRw6 homozygous and expressed HLA A2, Bw44, CW5, DRw6 and 16.23+. The monoclonal antibody secreted by S2 defines an epitope apparently restricted to DRQ13. Together with antibodies expressed by 16/23 (Sigma Catalogue number. 99043001) and S5 (Sigma Catalogue number. 99061827) they are useful tools for the biochemical dissection of DRw6 related epitopes. S2 has been analysed in International Histocompatibility Workshop (IOWS 3021, II WS 0826). The cells have been eradicated from mycoplasma before depositing at ECACC. The cells have a doubling time of about 36 hours.

Immunogen

B cell line Sch HLA A2, B44, C5 DR13

Application

Serological and biochemical analysis of class II products encoded by HLA-DRw6 haplotype

Culture Medium

RPMI 1640 + 2mM glutamine + 10% FBS

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml, 5% CO₂; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.