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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
P3x63Ag8.653
Reactivity
Anti-HLA-DR3 and DR52a
technique(s)
cell culture | mammalian: suitable
isotype
IgG3
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse x Mouse hybridoma
Cell Line Description
The cytotoxic monoclonal antibody 16.23 was obtained from a BALB/c mouse immunised with a single i.p. injection of the human melanoma cell line Mel Juso. Mouse spleen cells were removed 3 days after immunisation and fused with the myeloma P3x63Ag8.653. The Juso B cell line was typed as HLA A1,2; B7,8; CW- ; DR2,3. The monoclonal antibody 16.23 detects the conformation-dependant DR3 epitope but was also reported to react with some HLA-Dw/DRw6 individuals. Together with antibodies S2 (Sigma Catalogue number. 99043002) and S5 (Sigma Catalogue number. 99061827) a useful tool for the biochemical dissection of DRw6 related epitopes has been establised. It has been analysed in the 9th International Histocompatibility Workshop (9W929). The hybridoma line has been eradicated from mycoplasma using Cibrobay before depositing at ECACC.
Immunogen
Human melanoma cell line Mel Juso
Application
Immunohistochemistry, Cytotoxicity, Immunoprecipitation
Culture Medium
RPMI 1640 + 2mM glutamine + 1mM NaP + 10% FBS
Subculture Routine
Maintain cultures between 3-9 x100,000 cells/ml; 5% CO2; 37°C
Other Notes
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