LB81
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
biological source
human unknown/unspecified
packaging
tube of 5 μg 98062327-DNA-5UG
pkg of vial of cells 98062327-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human lymphoblastoid, Xeroderma pigmentosum heterozygote, EBV-transformed
Cell Line Description
Established by EBV transformation of mononuclear cells from a peripheral blood sample from a 37 year old female patient. The patient was shown to be heterozygote for Xeroderma pigmentosum complementation group G, a rare, autosomal recessive disease, and is the mother of two children with XP (cell line LB83, Sigma Catalogue number. 98062328 and LB82).
Application
Toxicity studies, hprt mutation analysis
Culture Medium
RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C. Cells form free floating aggregates.
Other Notes
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