LB58

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: human blood
• packaging: tube of 5 μg 98062325-DNA-5UG; pkg of vial of cells 98062325-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblastoid
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human lymphoblastoid, Ataxia telangiectasia, EBV-transformed

Cell Line Description

Established by EBV transformation of mononuclear cells from the peripheral blood of a 13 year old female with Ataxia telangiectasia (AT) complementation group C. AT is a rare autosomal recessive disorder. Lymphocytes have been shown to be hypersensitive to cytotoxic and clastogenic effects of gamma and X rays. T cells from the same original sample have been used for studies of mutant frequency at the hprt locus, with cells being described as Donor 58 or Patient 2 (see Lancet 1985). Fibroblasts derived from the same individual were reported to have RNA and genomic DNA sequence changes (del, 9nt).

Application

hprt mutation studies, toxicity assays

Culture Medium

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO₂, 37°C. Cells form floating aggregates.

Other Notes

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