LB443
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
biological source
human blood
packaging
tube of 5 μg 98062322-DNA-5UG
pkg of vial of cells 98062322-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human lymphoblastoid, trichothiodystrophy, EBV-transformed
Cell Line Description
Established by EBV transformation of mononuclear cell from a peripheral blood sample from a 4 year old male patient. The patient was diagnosed with trichothiodystrophy (TTD), a syndrome connected to deficiency in excision repair of ultraviolet radiation induced DNA damage. Studies with fibroblasts from the same patient, known as TTD4BR, have been published.
Application
Study of DNA repair deficiencies
Culture Medium
RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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