LB42
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
biological source
human blood
packaging
tube of 5 μg 98062313-DNA-5UG
pkg of vial of cells 98062313-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human lymphocytes, normal, EBV-transformed
Cell Line Description
Derived from a 58 year old normal male. The mononuclear cells have been used as a normal control for DNA repair deficiency studies, effect of UV irradiation and hprt mutation analysis. They have subsequently been transformed with EBV.
Application
DNA repair studies
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11
D13S317: 8
D16S539: 11,12
D5S818: 11
D7S820: 10,12
THO1: 7,9
TPOX: 8,11
vWA: 14,16
CSF1PO: 11
D13S317: 8
D16S539: 11,12
D5S818: 11
D7S820: 10,12
THO1: 7,9
TPOX: 8,11
vWA: 14,16
Culture Medium
RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C. Cells form floating aggregates.
Other Notes
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