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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
P3X63Ag8
Reactivity
Anti-annexin II, human
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Not specified
Cell Line Description
The hybridoma B1D6 was obtained by immunising BALB/c mice with human placental extract followed by intravenous injections with purified Fc gamma-receptor (receptor for the Fc portion of immunoglobulins, FcR). Spleen cells were then fused with the myeloma P3X63Ag8 to establish B1D6 secreting IgG1, kappa, monoclonal antibodies. This antibody has been shown to stain FcR-positive areas in sections of placental tissues, inhibited the haemadsorption of placental tissue of erythrocytes sensitised with IgG antibodies and the agglutination of these erythrocytes by FcR.
Immunogen
Human placenta FcR
Application
FcR studies
Culture Medium
RPMI 1640 + 2 mM glutamine + 10% FBS
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37 °C
Other Notes
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