IgG-6A6
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 97040309-DNA-5UG
pkg of vial of cells 97040309-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/0
Reactivity
Anti-VLDL receptor
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
BALB/c mouse spleen x Sp2/0
Cell Line Description
IgG-6A6 secretes a mouse monoclonal antibody that binds to the cytoplasmic domain of the very low density lipoprotein (VLDL) receptor. The hybridoma was produced by intraperitoneal injection of a peptide corresponding to the 10 carboxy-terminal amino acids of the VLDL receptor (SVVSTDDDLA) being coupled to keyhole limpet hemocyanin. BALB/c mice spleen cells were then fused with Sp2/0 mouse myeloma for the detection of VLDL receptor in various tissues.
Immunogen
VLDL peptide coupled to keyhole limpet hemocymin
Application
Immunoblot analysis of distribution and regulation of the VLDL receptor in animal tissues and cultured cells.
Culture Medium
DMEM + 2mM glutamine + 10% FBS
Subculture Routine
Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO2; 37°C; media renewal every 2-3 days
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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