55-6

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Mouse Hybridoma
• packaging: tube of 5 μg 97040308-DNA-5UG; pkg of vial of cells 97040308-1VL
• ImmunoDon: Mouse BALB/c spleen
• Myeloma: Sp2/0-Ag14
• Reactivity: Anti-HIV-1 gp120
• technique(s): cell culture | mammalian: suitable
• isotype: IgG2a
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse x Mouse hybridoma

Cell Line Description

The hybridoma cell line 55-6 produces antibodies reacting with the Human Immunodeficiency Virus I (HIV-1) envelope protein, gp120. Spleen cells from BALB/c mice that had been sequentially immunised with recombinant gp120(rgp120) from three different nonglycosylated isolates of HIV-1 (IIIB,SF2 and Z6) have been fused with Sp2/0-Ag14 myeloma cells. The antibody binds rgp120 from the III B isolate and reacts weakly with gp120 from the MN strain, both shown by ELISA. No cross blocking of any other antibodies in the developed panel was detected, thus indicating that 55-6 binds to a unique site on gp120IIIB. In peptide recognition assays it was shown to bind a synthetic peptide 105-117 (in the CI region) of gp120.

Immunogen

rgp 120

Application

gp120 assays

Culture Medium

RPMI 1640 + 2mM glutamine + HT + 10% FBS

Subculture Routine

Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO₂;37ºC

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.