CAR-2/3F4

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Mouse Hybridoma
• packaging: tube of 5 μg 97011602-DNA-5UG; pkg of vial of cells 97011602-1VL
• ImmunoDon: BALB/c mouse spleen
• Myeloma: P3X63Ag8.653
• Reactivity: Anti-Thioredoxin, human
• technique(s): cell culture | mammalian: suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

BALB/c mouse spleen x Mouse P3X63Ag8.653

Cell Line Description

CAR-2/3F4, also known as 2/3 F4, was formed by fusing P3X63Ag8-653 mouse myeloma cells with spleen cells from a BALB/c mouse immunised six times with 20μg human thioredoxin (TRX) at monthly intervals. Human TRX was cross-linked to Keyhole Limpet Haemocyanin for this purpose. The antibody is directed against monomeric and polymeric forms of human TRX and shows no cross-reactivity to bacterial TRX or human glutaredoxin. CAR-2/3F4 has been applied as capture antibody in a chemiluminescent detection assay for free human TRX. CAR-XP10 (Sigma Catalogue number. 97011601) can be used as second antibody in this assay.

Immunogen

Human Thioredoxin cross linked to Keyhole Limpet Haemocyanin

Application

Immunohistochemistry, Western blots

Culture Medium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 15% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 2-9 x 100,000 cells / ml; 5% CO₂; 37°C; start culture on mouse macrophage feeder layer

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.