CAR-XP10

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Mouse Hybridoma
• packaging: tube of 5 μg 97011601-DNA-5UG; pkg of vial of cells 97011601-1VL
• ImmunoDon: BALB/c mouse spleen
• Myeloma: P3X63Ag8.653
• Reactivity: Anti-Thioredoxin, human (monomeric and polymeric forms)
• technique(s): cell culture | mammalian: suitable
• isotype: IgG2a
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

BALB/c mouse spleen x Mouse P3X63Ag8.653

Cell Line Description

The hybridoma cell line CAR-XP10, also known as XP10, was produced by fusing P3X63Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunised six times with 20μg human thioredoxin (TRX) at monthly intervals. Human thioredoxin was cross-linked to Keyhole Limpet Haemocyanin for this purpose. The secreted antibodies react with monomeric and polymeric forms of human TRX and show no cross-reactivity to bacterial TRX or human glutaredoxin. CAR-XP10 has been used as second antibody in chemiluminescent detection assays for full human TRX. CAR-2/3F4 (Sigma Catalogue number. 97011602) can be applied as capture antibody.

Immunogen

Human Thioredoxin cross-linked to Keyhole Limpet Haemocyanin

Application

Immunohistochemistry, Western blots

Culture Medium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 15% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells / ml; 5% CO₂; 37°C; start culture on mouse macrophage feeder layer

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.