The best way to make this determination is to perform a kill curve of Blasticidin S HCl. Here as an example of the Kill Curve protocols: https://www.toku-e.com/content/product-documents/Protocols_Blasticidin_S_HCl_Kill_Curve_Protocol.pdf
Ri-1
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96090512, human blood, Lymphoblastoid
Synonym(s):
Ri-1 Cell Line
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
human blood
packaging
tube of 5 μg 96090512-DNA-5UG
pkg of vial of cells 96090512-1VL
growth mode
Suspension
karyotype
92XXXX/69XXX,t(1,2)(p32,q34)t(2,8)(p11,q24)t(14,18
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian B-cell lymphoma
Cell Line Description
The cell line Ri-1 has been established from the peripheral blood of a 48-year-old female patient with B-cell lymphoma. This EBV-negative cell line with 14q+ abnormality was shown to be at an intermediate stage of B-cell differentiation. Ri-1 expresses surface and cytoplasmic immunoglobulin (IgM kappa), HLA-DR, CD20, CD38 and FMC7. Rearranged heavy-light chain genes and one rearranged kappa light-chain gene was detected as well as a kappa and lambda light-chain gene in germ-line configuration.
Application
Study of B-cell lymphoma-leukaemia
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cell growth is very slow after resuscitation; large amounts of debris present.
Other Notes
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May I know the concentration of blasticidin used for selection when introducing a gene into cells, such as the NF-κB gene into a cell line like RI-1? Thanks.
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