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description
Mouse B cell hybridoma
shipped in
dry ice
storage temp.
−196°C
General description
The B cell hybridoma B9 has been cloned from the parent line B13.29 and is highly dependent on IL-6 for growth. Cells also proliferate in response to high concentrations (approximately 1000 pg/ml) of murine IL-4 and human IL-13. The cells have been used in bioassays for IL-6 of human, bovine, horse, pig, dog, rat, mouse and seal origin. For routine culture, cells are grown in 10-100 units/ml IL-6. An IL-6 assay can be carried out in a flat bottom microtiter plate in 200μl. 5000 B9 cells/well are cultured and labeled at 68-72 hours with 7.4kBq 3H-Thymidine. Samples to be tested are titrated and related to a standard preparation; 1U/ml is the concentration that leads to half-maximal 3H-Thymidine incorporation.
Application
Subculture: Maintain cultures between 1,000 - 900,000 cells/ml; 5% CO2; 37°C.
Recommended culture medium: IMDM or RPMI 1640 + 2mM Glutamine + 50μM 2-Mercaptoethanol (2ME) + 50pg/ml recombinant human IL-6 (diluted and stored in PBS containing 1% BSA) + 5% FBS (no Glutamine required with IMDM)
Recommended culture medium: IMDM or RPMI 1640 + 2mM Glutamine + 50μM 2-Mercaptoethanol (2ME) + 50pg/ml recombinant human IL-6 (diluted and stored in PBS containing 1% BSA) + 5% FBS (no Glutamine required with IMDM)
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