Ea.4
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
biological source
insect larvae
packaging
tube of 5 μg 96073116-DNA-5UG
pkg of vial of cells 96073116-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Round
products
Complex glycoproteins
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Saltmarsh caterpillar Estigmena acrea (Subclone 4)
Cell Line Description
This cell line can be used to produce complex glycoproteins, and has been demonstrated as having more extensive N-glycosylation compared to other insect cell lines used for the expression of recombinant proteins. Ea.4 is susceptible to infection by Baculovirus, which can be used as a vector for recombinant protein synthesis.
Application
Recombinant complex glycoprotein production, Baculovirus infection.
Culture Medium
TiterHigh™ SF Insect Medium (Sigma I5408) (no supplements)
Subculture Routine
Maintain cultures between 2x100,000 and 1x1,000,000 cells/ml. Doubling time 36 hours. Grow at 26°C. Freeze in 90% HI-FBS plus 10% glycerol.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Legal Information
TiterHigh is a trademark of Sigma-Aldrich Co. LLC
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