Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.
OE33 Cell Line human
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96070808, human esophagus, Epithelial
Synonym(s):
JROECL33, JROECL33 Cells, OE-33 Cells
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
human esophagus
packaging
tube of 5 μg 96070808-DNA-5UG
pkg of vial of cells 96070808-1VL
growth mode
Adherent
karyotype
Aneuploid
morphology
Epithelial
products
Not specified
receptors
MHC class I, ICAM-1
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian oesophageal carcinoma
Cell Line Description
The cell line OE33, also known as JROECL33, was established from the adenocarcinoma of the lower oesophagus (Barrett′s metaplasia) of a 73 year old female patient. The tumour was identified as pathological stage IIA (UICC) and showed poor differentiation. OE33 express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice.
Application
OE33 cell line has been used to study the effect of microRNA-MiR-193b on autophagy and cell death on these cells.[1] It has also been used to study the sensitivity of the cells to anti-c-Met monoclonal antibody ABT-700.[2]
Study of oesophageal cancer
Subculture Routine
Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C. Initially these cells grow slowly and can take up to 7 days until ready for the next split, 50% media changes will be necessary every 2-3 days (i.e. replacing half the old medium with fresh).
Other Notes
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How do I handle Cell Line frozen cells upon arrival?
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What passage are my Cell Line cells?
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If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).
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Are the Cell Line cells tested for viral pathogens?
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HPA Cultures does not perform any viral testing on the cell lines.
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What medium should I use when I thaw the Cell Line cells?
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The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.
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Are my Cell Line cells mycoplasma tested?
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ECACC routinely tests all manufactured cell banks for mycoplasma.
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Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?
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If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.
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How many Cell Line cells are in the vial?
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Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule. For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.
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