EMT6/CPR
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
mouse unknown/unspecified
packaging
tube of 5 μg 96042318-DNA-5UG
pkg of vial of cells 96042318-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse mammary, drug-resistant
Cell Line Description
The cisplatin-resistant variant of the mouse mammary tumour cell line EMT6/P (Sigma Catalogue number. 96042344) was derived by continuous in vitro exposure to increasing concentrations of cisplatin over a period of 6 weeks. EMT6/CPR was shown to express 2.5-fold resistance to cisplatin as compared with the sensitive parent line. Development of drug resistance was accompanied by a 1.7-fold increase in cellular glutathione (GSH). Growth for 4 weeks in the absence of cisplatin was reported to lead to loss of resistance phenotype and elevated GSH levels in the resistant cell line EMT6/CPR.
Application
Drug-resistance studies
Culture Medium
MEM(EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 0.25μg/ml, cisplatin + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37ºC. It is recommended to culture the cells without drugs upon resuscitation until the first passage.
Other Notes
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