UM-UC-3

96020936, human bladder, Epithelial

• UNSPSC Code: 41106514

Properties

• Product Name: UM-UC-3, 96020936
• biological source: human bladder
• growth mode: Adherent
• karyotype: Hypertriploid. The modal chromosome number was 80, occurring in 42% of cells. Cells with 78 chromosomes also occurred at a high frequency.
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Bladder Transitional Cell Carcinoma

Cell Line Description

Derived from a urinary bladder transitional cell carcinoma from a human male. These cells are tumourigenic in nude mice when 10E7 cells are inoculated subcutaneously. Hypertriploid. The modal chromosome number was 80, occurring in 42% of cells. Cells with 78 chromosomes also occurred at a high frequency. The rate of cells with higher ploidies was 2.5%. There were 30 or more marker chromosomes in each cell. They included der(1)t (1;?) (p32;?), ?t(1p5p), i(3q), t(7q14q), ?t(2p3p) and others. The X and N3 had single copy per cell, and others were generally two or three copies per cell.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8
D16S539: 8,9
D5S818: 12
D7S820: 8,9
THO1: 6,9
TPOX: 10
vWA: 17

Culture Medium

EMEM EBSS + 2mM Glutamine + 0.1mM Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) + 1.5g/L sodium bicarbonate + 1.0mM sodium pyruvate.

Subculture Routine

Split sub-confluent cultures (70-80 %) 1:4 to 1:10 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 37 C; 5% CO₂.

Other Notes

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