Derived from a fibroma of a 14 month old male Mongolian gerbil (Meriones unguiculatus). Susceptible to vesicular stomatitis virus(Indiana), herpes simplex virus and vaccinia virus. Resistant to poliovirus 1 and echoviruses.
Culture Medium
Medium 199 with Hanks? BSS + 2mM Glutamine 20% Foetal Bovine Serum (FBS)
Subculture Routine
Split sub-confluent cultures (70-80 %) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 37 C; 5% CO2.
Other Notes
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