ED17
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 95110627-DNA-5UG
pkg of vial of cells 95110627-1VL
ImmunoDon
Mouse spleen cells
Myeloma
Sp2/0
Reactivity
Anti-myeloid antigen 55kDa, rat
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Not specified
Cell Line Description
The ED17 hybridoma cell line secretes antibodies reactive against a 55 kDa membrane antigen on tissue macrophages. ED17 reacts strongly with red pulp macrophages in the spleen and Langerhans cells in the skin as well as peritoneal macrophages. The antibody also recognises rat SIRP (personal communication).
Immunogen
Wistar rat spleen
Application
Immunohistochemistry, cytochemistry, FACS
Culture Medium
RPMI 1640 + 2 mM glutamine + 10% FBS
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37 °C
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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