P-tyr-1
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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95092903-DNA-5UG
$402.00
1 VIAL
$981.00
$402.00
Usually ships in 1 week. (Orders outside of Europe & Scandinavian countries, allow an additional week for delivery
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95092903-DNA-5UG
$402.00
1 VIAL
$981.00
About This Item
UNSPSC Code:
41106514
$402.00
Usually ships in 1 week. (Orders outside of Europe & Scandinavian countries, allow an additional week for delivery
biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 95092903-DNA-5UG
pkg of vial of cells 95092903-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
P3X63Ag8.653
Reactivity
Anti-Phosphotyrosine
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse P3X63Ag8.653 x Mouse BALB/c spleen
Cell Line Description
The hybridoma cell line P-tyr-1 was produced by fusing P3X63Ag8.653 mouse myeloma cells with spleen cells from a female BALB/c mouse immunised with aminobenzyl phosphonate. Aminobenzyl phosphonate is a structural analogue of the side-chain in phosphotyrosine, and was coupled to hemocyanin (antibody P-HC) for immunisation. The secreted IgG1 antibodies react with phosphotyrosine and will detect phosphotyrosine-containing proteins by immunoprecipitation and Western blot analysis. The antibody does not react with phosphoserine or phosphothreonine. P-tyr-1 was tested for Bovine Viral Diarrhoea Virus (BVDV) at ECACC and found to be negative.
Immunogen
Aminobenzyl phosphonate
Application
Detection of phosphotyrosine-containing proteins
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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