HR5-CL11
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
human cervix
packaging
tube of 5 μg 95091411-DNA-5UG
pkg of vial of cells 95091411-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human cervix carcinoma, HeLa derivative, transformed
Cell Line Description
HR5-CL11 cells were obtained after transfection of HeLa cells with plasmids coding for a transcriptional transactivator and transferring G418 and hygromycin resistance. This tetracycline-controlled transactivator (a fusion between the tet repressor and the activating domain of the herpes simplex virus VP16 transcription activation domain) can stimulate the expression of a reporter gene up to 100,000 fold. Subsequently these cells were stably transfected with a luciferase reporter unit. Luciferase expression in the HR5-CL11 cells is highly stimulated by doxycycline.
Application
Gene expression and activation studies
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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