Hepa-1c1c7

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95090613, mouse liver, Epithelial

• UNSPSC Code: 41106514

Properties

• biological source: mouse liver
• packaging: tube of 5 μg 95090613-DNA-5UG; pkg of vial of cells 95090613-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Aryl hydrocarbon
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse hepatoma

Cell Line Description

Hepa-1c1c7 was cloned from the cell line Hepa-1cl which was obtained from Hepa-1. Hepa-1 was derived from the BW7756 hepatoma that developed in a C57L mouse. Hepa-1c1c7 cells express the aryl hydrocarbon (Ah) receptor and were shown to be highly inducible for cytochrome P450IA1. A variety of mutants from this cell line have been established.

Application

Study of Ah receptors, P450IA1 regulation

Culture Medium

α MEM without nucleosides + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.