MES-SA/Dx-5

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95051031, human uterus, Epithelial

• UNSPSC Code: 41106514
• eCl@ss: 32190102

Properties

• biological source: human uterus
• packaging: tube of 5 μg 95051031-DNA-5UG; pkg of vial of cells 95051031-1VL
• growth mode: Adherent
• karyotype: Modal no. 47 (parent line MES-SA-45). Reported karyotype 47,XX,12qt,22q-t(5q,6p),-3,-4,-7,+t(3q?,7q),Mar1,+mar2,+Mar3
• morphology: Epithelial
• products: High levels of Mdr1 mRNA and P-glycoprotein
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• Storage temp.: −196°C

Description

Cell Line Origin

Human uterus sarcoma

Cell Line Description

The multi drug-resistant cell line MES-SA/Dx5 was derived from the human uterine sarcoma cell line MES-SA (Sigma Catalogue number. 95051030) which was originally obtained from a tumour from a 56-year-old Caucasian female at the time of hysterectomy. The Dx5 variant exhibits a 100-fold resistance to doxorubicin and has a reported doubling time of 30 hours. The two additional marker chromosomes indicate clonal selection during drug selection. MES-SA/Dx-5 cells exhibit marked cross-resistance to a number of chemotherapeutic agents (including daunorubicin, dactinomycin, vincristine, taxol, colchicine) and moderate cross-resistance to mitomycin C and melphalan. Cross resistance to bleomycin, cisplatin, carmustine, 5-fluorouracil or methotrexate was not observed.

Application

Tumour toxicity assays and multi-drug-resistant studies.

Culture Medium

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.03% EDTA; 5% CO₂; 37°C. Tap side of flask to dislodge cells.

Other Notes

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