HSG (HeLa derivative)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: human cervix
• packaging: tube of 5 μg 95031024-DNA-5UG; pkg of vial of cells 95031024-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: EGF, TGF-beta
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human cervix carcinoma

Cell Line Description

The cell line HSG was originally thought to be established from a human submandibular gland from a 54 year old Japanese male. The cell line has since been found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

Culture Medium

MEM +2mM Glutamine +1% Non Essential Amino Acids (NEAA) + 10% New Born Calf Serum (NBCS).

Subculture Routine

Split confluent cultures 1:4 to 1:10 ie seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂, 37°C

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.