H5

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: rat liver
• packaging: tube of 5 μg 94101905-DNA-5UG; pkg of vial of cells 94101905-1VL
• growth mode: Adherent
• karyotype: Modal no. 52 (50-55)
• morphology: Fusiform
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Rat hepatoma

Cell Line Description

H5 is a subclone of H4-II-E-C3 (Sigma Catalogue number. 85061112) which originated from the Reuber H-35 hepatoma induced by chemical carcinogenesis in an A x C rat. The dedifferentiated H5 cells express high levels of glucose-6-phosphate dehydrogenase and exhibit a population doubling time of 12 hours. Differentiated variants are H4IIEC3 (85061112), Fao and others.

Application

Toxicity studies; negative control for differentiated hepatoma

Culture Medium

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:50 to 1:100 i.e. seeding at 1-2x1,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.