G

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: rat prostate
• packaging: tube of 5 μg 94101453-DNA-5UG; pkg of vial of cells 94101453-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Androgen sensitive
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Rat Dunning R-3327,prostate adenocarcinoma

Cell Line Description

Derived from a Dunning R-3327 rat with prostatic adenocarcinoma. G is one of a group of variants which developed by a single in vivo passage. It is androgen responsive, moderately fast growing and poorly differentiated. Using this system of rat prostatic adenocarcinomas as a model, it has been demonstrated that prostatic cancer cells can become unstable at any time resulting in the addition of cytogenetically and phenotypically novel clones of cancer cells to the cancer population.

Culture Medium

RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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