AT-3.1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 94101451

• UNSPSC Code: 41106514

Properties

• biological source: rat skin
• packaging: tube of 5 μg 94101451-DNA-5UG; pkg of vial of cells 94101451-1VL
• growth mode: Adherent
• karyotype: Hyperaneuploidy
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Rat dorsal prostatic adenocarcinoma

Cell Line Description

AT-3.1 is a subclone of the AT-3 cell line being one of a series of related variants originating from a single spontaneous adenocarcinoma in the dorsal lobe of the prostate of an aged male inbred Copenhagen rat. AT-3.1 was derived from an anaplastic tumour with a high rate of metastases and is androgen-independent.

Application

Carcinogenesis studies, cytogenetic analysis.

Culture Medium

RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 2-4 x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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