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product name
AT-1, 94101449
biological source
rat prostate
growth mode
Adherent
karyotype
Modal no. 62
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat Dunning R-3327,dorsal prostatic adenocarcinoma
Cell Line Description
Derived from a spontaneous Dunning R-3327 rat prostatic cancer. The cells have been shown to be anaplastic with low metastatic ability and non-responsive to androgen.
Culture Medium
RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 2-4 x1000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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