M. dunni (Clone III8C)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94101211, mouse tail, Fibroblast-like

• UNSPSC Code: 41106514

Properties

• biological source: mouse tail
• packaging: tube of 5 μg 94101211-DNA-5UG; pkg of vial of cells 94101211-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast-like
• products: Not specified
• receptors: Ecotropic Murine leukemia virus (MuLV)
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse tail, normal fibroblast

Cell Line Description

Derived from the tail tissue of a normal female mouse, Mus dunni. The cells were cloned at passage 14 using Tererasiki plates. M. dunni (Clone III8C) supports the replication of all four classes of murine leukaemia viruses (MuLV); ecotropic, amphotropic, xenotropic and mink cell focus-forming viruses (MCF), showing cytopathic effect. The cells lack most endogenous murine leukaemia virus-related sequences. They are resistant to infection by Moloney MuLV, due to post-translational modifications of the ecotropic MuLV receptor. Confluent cultures will remain more firmly attached in RPMI 1640 or DMEM + 10% FBS during virus assays.

Application

Detection of replication-competent retroviruses in gene therapy.

Culture Medium

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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