Colo 794
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94072237, human skin, Not specified
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
human skin
packaging
tube of 5 μg 94072237-DNA-5UG
pkg of vial of cells 94072237-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human melanoma
Cell Line Description
Colo 794 was established from a 14 year old male patient with a malignant melanoma. The melanoma showed rapid growth and metastases in the patient and the biopsy was taken before therapy from a subcutaneous metastasis. The cell line was eradicated from mycoplasma at ECACC.COLO 794 and COLO 800 (Sigma Catalogue number 93051123) were both derived from the same 14 year old male This has been confirmed by STR profiling.
Application
Cancer studies
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 11
D5S818: 11
D7S820: 10,11
THO1: 6
TPOX: 11
vWA: 16,20
CSF1PO: 10
D13S317: 11,13
D16S539: 11
D5S818: 11
D7S820: 10,11
THO1: 6
TPOX: 11
vWA: 16,20
Culture Medium
RPMI 1640+ 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C; cells can show extensive blebs
Other Notes
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