GRL101 (MIX)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: hamster ovary
• packaging: tube of 5 μg 94071258-DNA-5UG; pkg of vial of cells 94071258-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: GRL101 - putative Lymnaea receptor
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Hamster Chinese ovary transfected with Lymnaea receptor GRL101

Cell Line Description

Cell line containing the receptor protein GRL101. The cDNA from the CNS of the mollusc Lymnaea stagnalis encoding a putative receptor has been isolated and analysed. The first repeat of DNA displays a high sequence similarity to the extracellular binding of the low density protein receptor, which binds and internalises cholesterol containing apolipoproteins. The MRNI receptor is predominantly expressed in a small number of neurons within the CNS and to a lesser extent in the heart. Contains a mixed culture of approximately 20 transfected clones.

Application

Studies in the newly characterised members of a putative class of G protein - linked receptors that directly transduce signals carried by large extracellular (lipo)protein (complexes) into neural events.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2 - 4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂, 37°C.

Other Notes

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