RAT-2
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
rat embryo
packaging
tube of 5 μg 94050409-DNA-5UG
pkg of vial of cells 94050409-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
Storage temp.
−196°C
Cell Line Origin
Rat embryo
Cell Line Description
RAT-2 was derived from a subclone of a 5′-bromo-deoxyuridine (BUdR)-resistant strain of the Fischer rat 3T3-like cell line RAT-1. RAT-2 lacks appreciable levels of nuclear thymidine kinase, is highly transfectable by exogenous DNA and is phenotypically normal.
Culture Medium
DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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