AtT-20/D16v-F2

94050406, mouse brain, Epithelial

• UNSPSC Code: 41106514
• biological source: mouse brain
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• relevant disease(s): cancer

Properties

• Product Name: AtT-20/D16v-F2, 94050406
• biological source: mouse brain
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse pituitary tumour

Cell Line Description

This F2 subclone was developed from original AtT-20, an andrenocorticotropic hormone (ACTH) secreting cell line established from murine pituitary tumour. These cells are readily transfected using a standard calcium phosphate protocol.

application

AtT-20/D16v-F2 has been used to study the role of the G-protein Rasd1 in hypothalamic response to neuronal activation.[1] It has also been used to study the effect of cAMP and glucocorticoid treatment on cAMP-responsive element binding protein 3 like 1 (CREB3L1).[2]

Culture Medium

DMEM + 2mM Glutamine + 10% FBS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:5 using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cell clumps must be broken up to prevent cells growing in suspension.

Other Notes

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