OVNIS

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: ovine thyroid
• packaging: tube of 5 μg 93122324-DNA-5UG; pkg of vial of cells 93122324-1VL
• morphology: Epithelial
• products: Thyroglobulin, plasminogen activators (t-PA and u-PA)
• receptors: Thyrotropin, insulin, hydrocortisone, Insulin Growth Factor (IGF)-I
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Ovine thyroid epithelial

Cell Line Description

Isolated from ovine thyroid and adapted to grow in low serum (0.1%) media.

Application

Regulation of thyroid cell metabolism

Culture Medium

Ham′s F12 + 121mg/l CaCl2(2H20) + 10μg/ml Insulin + 10nM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml Glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 10mU/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 0.1%Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO₂; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.