SK MES 1

93120837, human lung, Epithelial

• UNSPSC Code: 41106514

Properties

• product name: SK MES 1, 93120837
• biological source: human lung
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian lung squamous carcinoma

Cell Line Description

Derived from the pleural effusion of a 65 year old Caucasian male with squamous cell carcinoma of the lung. HLA = A3, B7, B27, w30.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 13
D5S818: 11
D7S820: 8
THO1: 6,9.3
TPOX: 8
vWA: 14

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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