R74

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: rat embryo
• packaging: tube of 5 μg 93120215-DNA-5UG; pkg of vial of cells 93120215-1VL
• growth mode: Adherent
• karyotype: Near diploid
• morphology: Fibroblast-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Rat embryonic fibroblasts RSV-33 transformed

Cell Line Description

The R74 line was derived by co-cultivation of 18 day-old Lewis rat embryonic fibroblasts with cells of a chicken positive for Rous sarcoma virus-33 (RSV-33). This cell population is clearly distinguishable from RT1 and R9 as it is characterised by the presence of fibroblastoid cells that tend to round up and form clusters of refractile cells. R74 is also tumourigenic in chickens and rats.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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