VPM 44

Reactivity: Anti-MHC class II DQβ chain, ovine, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 93113033

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Mouse Hybridoma
• packaging: tube of 5 μg 93113033-DNA-5UG; pkg of vial of cells 93113033-1VL
• ImmunoDon: BALB/c mouse spleen
• Myeloma: NS0
• Reactivity: Anti-MHC class II DQβ chain, ovine
• technique(s): cell culture | mammalian: suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse NS0 x Mouse BALB/c spleen

Cell Line Description

The antibody is directed against MHC class II molecules on cell surface and on immunoblots. It also reacts with polymorphic determinant and is therefore subgroup specific.

Immunogen

Ovine MHC class II

Application

Identifying and characterising sheep MHC class II molecules

Culture Medium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 1-2x100,000 cells/ml; 5% CO₂; 37°C. Saturation density reached at 3-9x100,000 cells/ml. (takes 3-4 days to reach 5x100,000)

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.