VPM 38

93113029, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., Reactivity: Anti-MHC class II α-chain, ovine

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Mouse Hybridoma
• packaging: tube of 5 μg 93113029-DNA-5UG; pkg of vial of cells 93113029-1VL
• ImmunoDon: BALB/c mouse spleen
• Myeloma: NS0
• Reactivity: Anti-MHC class II α-chain, ovine
• technique(s): cell culture | mammalian: suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse NS0 x Mouse BALB/c spleen

Cell Line Description

The antibody cross-reacts with bovine and human MHC class II. It reacts with MHC class II molecules on cell surfaces and on immunoblots. It reacts very weakly with ovine DQ L cell transfectants by immunofluorescence, but does not react with DQ blots and therefore is classified as strongly DR α specific, but weakly cross-reactive with DQ α.

Immunogen

Ovine MHC class II

Application

Quantification of DQ and DR expression on T cell subsets in adult and foetal peripheral blood, afferent lymph, lymph node and efferent lymph.

Culture Medium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 1-2x100,000 cells/ml; 5% CO₂; 37°C. Saturation density is reached at 3-9x100,000 cells/ml.

Other Notes

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