LR-3
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., Reactivity: Anti-Heat Stable Antigen, mouse, 93062221
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
biological source
Rat x Mouse Hybridoma
packaging
tube of 5 μg 93062221-DNA-5UG
pkg of vial of cells 93062221-1VL
ImmunoDon
Rat Wistar spleen cells
Myeloma
P3X63Ag8.653
Reactivity
Anti-Heat Stable Antigen, mouse
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat Wistar spleen x Mouse P3X63Ag8.653
Cell Line Description
LR-3 hybridoma cells were produced by immunisation of Wistar rats with bone marrow from Mesocestoides cortii infected BALB/c cells. Subsequently rat spleen cells were fused with P3X63Ag8.653 myeloma cells.
Immunogen
Bone marrow from Mesocestoides cortii infected BALB/c mice
Application
Detection of murine B cells, mAb appear to stimulate B cell proliferation. Detection of HSA by immunopricipitation.
The isotype of the monoclonal antibody produced by this cell line is rat IgM.
Culture Medium
DMEM + 2mM glutamine + 5-10% Foetal Bovine Serum (FBS), feeder layer improves growth
Subculture Routine
Maintain cultures between 1-2x100,000 cells/ml; 5% CO2; 37°C
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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