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A1H4

93062220, Reactivity: Anti-Madin-Darby canine kidney cell E-cadherin (uvomorulin), NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

UNSPSC Code:
41106514
Pricing and availability is not currently available.

biological source

Mouse x Mouse Hybridoma

packaging

tube of 5 μg 93062220-DNA-5UG
pkg of vial of cells 93062220-1VL

ImmunoDon

BALB/c mouse spleen

Myeloma

P3X63Ag8.653

Reactivity

Anti-Madin-Darby canine kidney cell E-cadherin (uvomorulin)

technique(s)

cell culture | mammalian: suitable

isotype

IgG1, kappa

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse P3X63Ag8.653 x Mouse BALB/c spleen

Cell Line Description

The antibody is directed against a 188kDa polypeptide present on the basolateral plasma membrane of Madin-Darby canine kidney (MDCK) cells. This polypeptide is the same as, or closely related to, the cell adhesion molecule uvomorulin.

Immunogen

Madin-Darby canine kidney (MDCK) cells

Application

Study of epithelial occluding barriers

Culture Medium

EMEM(EBSS) + 2mM glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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