4.6.5a114K
93062218, Reactivity: Anti-Madin-Darby canine kidney cells
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/0 Ag-14
Reactivity
Anti-Madin-Darby canine kidney cells
technique(s)
cell culture | mammalian: suitable
isotype
IgG1, kappa
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse Sp2/0 Ag-14 x Mouse BALB/c spleen
Cell Line Description
The antibody is directed against a 144kDa protein present predominantly on the apical, but also on the basolateral surfaces of Madin-Darby canine kidney cells of the MDCK cell line.
Immunogen
Basolateral membrane fraction from dog intestine
Application
Study of cell surface polarity development
Culture Medium
EMEM(EBSS) + 2mM glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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