J45.01
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
human blood
packaging
tube of 5 μg 93031145-DNA-5UG
pkg of vial of cells 93031145-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Interleukin 2 (IL-2) in response to calcium ionophores and phorbol ester
receptors
TcR, CD2, CD28, CD45 (low)
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human acute T cell leukaemia
Cell Line Description
Derived from Jurkat E6-1 clone (Sigma Catalogue number. 88042803) after gamma irradiation and complement-mediated lysis with anti CD45. Low expression of CD45 (5-8% compared to parent line) results in defective signal transduction through TcR and the accessory molecule, CD2. Interleukin 2 (IL-2) is produced in response to calcium ionophore and phorbol ester stimulation, but it does not produce IL-2 in response to TcR or CD2 stimulation.
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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