Z2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., Reactivity: Anti-IgG2a, mouse, 93022522

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Mouse Hybridoma
• packaging: tube of 5 μg 93022522-DNA-5UG; pkg of vial of cells 93022522-1VL
• ImmunoDon: BALB/c mouse spleen
• Myeloma: Sp2/0
• Reactivity: Anti-IgG2a, mouse
• technique(s): cell culture | mammalian: suitable
• isotype: IgG2b
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse Sp2/0 x Mouse BALB/c spleen

Cell Line Description

The immunisation protocol involved repeated intranasal infection with a single i.v. injection before fusion. Produces IgG2b antibody which binds murine IgG2a of "a" and "b" allotype, Fc region. There is no cross-reactivity with A/PR/8/34 influenza virus, DNP, NP, DNA or cytoskeletal proteins.

Immunogen

A/PR/8/34 mouse pathogenic human influenza virus

Application

Differential detection of complexed IgG2a in the presence of native IgG2a, allotype detection or quantitation.

Culture Medium

RPMI 1640 or DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS) (can be adapted to 1% Foetal Bovine Serum (FBS))

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

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