V79-4

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93010723, hamster lung, Fibroblast

• UNSPSC Code: 41106514

Properties

• biological source: hamster lung
• packaging: tube of 5 μg 93010723-DNA-5UG; pkg of vial of cells 93010723-1VL
• growth mode: Adherent
• karyotype: 2n = 22
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Hamster Chinese lung

Cell Line Description

Derived from the lung tissue of a male Chinese hamster. The cells exhibit a high plating efficiency and a doubling time of 12-14 hours. Originally used in the discovery of X-ray induced damage and recovery in mammalian cells. This is a clone of cell line V79 (Sigma Catalogue number. 86041102).

Application

Mutagenesis and mammalian cell genetic studies

Culture Medium

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:6 to 1:14 i.e. seeding at 3x10,000-1x100,000 cells/cm²; 5% CO₂; 37°C using 0.25% trypsin/EDTA.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.