RENT ts3.1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: rat endometrium
• packaging: tube of 5 μg 92092516-DNA-5UG; pkg of vial of cells 92092516-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Cuboidal
• products: Not specified
• receptors: Glucocorticoid
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Rat Sprague-Dawley, primary endometrial, SV40 T antigen transformed

Cell Line Description

Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat, transformed with a ts mutant of SV40 T antigen (tsA58). Cells express vimentin, CSF-1 (at 39°C), desmin, collagen type III and connexin 43.

Culture Medium

DMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 34°C. Doubling time 48 hours. Cells show continuous growth at 34°C and growth arrest at 39°C.

Other Notes

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