RENT 4
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
biological source
rat endometrium
packaging
tube of 5 μg 92092512-DNA-5UG
pkg of vial of cells 92092512-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Spindle shaped
products
Not specified
receptors
Glucocorticoid
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat Sprague-Dawley, primary endometrial, SV40 large T antigen transformed
Cell Line Description
Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. Cells carry vimentin, connexin 43, collagen type III and proenkephalin markers. The transforming agent was SV40 large T antigen.
Culture Medium
DMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Doubling time 48 hours.
Other Notes
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