Product Name
ECV304, 92091712
biological source
human bladder
growth mode
Adherent
karyotype
High trisomic (modal no. 80)
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human urinary bladder carcinoma
Cell Line Description
Using new PCR based single locus fingerprint techniques, it was discovered that this cell line is identical to T-24. Because T-24 was established in 1970 compared to 1984 for ECV304 it is assumed that ECV304 is the false cell line. Originally, ECV304 was described by the originator as spontaneously transformed cell line derived from an umbilical cord from a new born Japanese female. Tumorigenic in Nude mice.
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 9
D5S818: 10
D7S820: 10,11
THO1: 6
TPOX: 8,11
vWA: 17
CSF1PO: 12
D13S317: 12
D16S539: 9
D5S818: 10
D7S820: 10,11
THO1: 6
TPOX: 8,11
vWA: 17
Culture Medium
Medium 199 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA. 5% CO2; 37°C.
Other Notes
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