ST
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92040221, Porcine testis, Not specified
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
Porcine testis
packaging
tube of 5 μg 92040221-DNA-5UG
pkg of vial of cells 92040221-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Porcine testis, foetal
Cell Line Description
The cell line was established in 1960 from the trypsinised testis of a normal 80-90 day old porcine foetus. The cells support the growth of various porcine parvo- and entero-viruses, and also pseudorabies virus. Also useful in the propagation and isolation of transmissible gastro-enteritis of swine.
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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